Label Free Real-Time PCR

Polymerase chain reaction (PCR) has been widely utilized for both basic biological research and medical applications. Fluorescence labeling is a common approach for monitoring real-time DNA amplification. However, fluorescence-reagent volume is one of the factors that could potentially cause bias quantification of DNA amplification in PCR. SAFEbiosense LLC is working on a label-free approach for real-time quantification of DNA amplification to address the issues in the conventional fluorescence-based approach.